After the liquid strain is cultivated, the
inoculation method directly affects the advantage of the liquid strain, and even relates to the success or failure of the production. There are a variety of inoculation methods in large-scale application liquids, each with its own characteristics. The following are introduced for your reference.
1. Material surface inoculation method
This is the most common method currently used. The inoculation is mostly bottled or bagged culture material, and there is a certain space at the top, such as the production of enoki mushrooms, tea tree mushrooms, black fungus, pleurotus eryngii mushroom bags, as well as the production of shiitake mushrooms, Coprinus comatus, and oyster mushrooms. When inoculating, try to spray the surface of the material with bacterial species. The germination and growth of the liquid species will occur quickly. The chance of contamination by early bacteria on the cover is very small. The chance of bacterial bag contamination is extremely low except for broken bags. Achieve zero pollution. The bacteria balls of this method grow well under the conditions of sufficient oxygen.
2. Insertion inoculation method with a pipette tip on the surface
Because of the surface inoculation method, the bacteria balls are easily blocked by the filtering effect of the culture material on the upper part of the material, so the growth of mycelium needs to grow from top to bottom, and cannot grow up and down in the material at the same time. If the tip of the inoculation gun is made into a tube and inserted into the material for inoculation, the bacteria balls will grow in the material at the same time, which can shorten the germination time by more than half. In this method, one should pay attention to the supply of oxygen in the material (not suitable for bagging), and the other should pay attention to the aseptic requirements of the inoculation environment to avoid contamination by the germs that do not germinate or the bacteria are brought into the gun head. For example, even if the mushroom sticks are inserted into the center of both ends during production, even if the mouth is not sealed, the strains in the middle section of the sticks are not easy to germinate. This method inoculates large quantities of mushrooms, Coprinus comatus, Flammulina velutipes, Pleurotus ostreatus and other strains in the low temperature season, and the finished product rate is 98% to 100%, which shortens the time by more than half.
3. The inoculation method of piercing the surface of the bag
Inoculation of long sticks from both ends can not shorten the cultivation time. Use a pipette tip from one or both sides of the bag to pierce 3~5cm deep and inoculate at the same time. The interval of piercing holes is determined according to the needs, such as mushrooms with 3~5 holes per stick. This method adjusts the amount of inoculation and the distance between the holes can achieve the purpose of shortening the cultivation time and reducing the contamination of bacteria. The disadvantage is that the amount of seeds used is large, and the inoculation amount of each hole is not less than 10mL. More than 20mL per hole.
4. Spray inoculation method inside the bag wall
Long bacteria sticks can also be pierced into the bag film with gun heads from both ends, spray the bacteria between the material and the plastic film (the liquid pressure is required to reach 9.8×100KPa or more), and press to disperse the bacteria after spraying. Seed, so that one side of the rod is covered with bacteria, and after the hypha grows 1~3cm, the hole can be pierced to increase oxygen. This method can cover a larger surface of the material, and the microporous pollution of the bag surface is small. The disadvantage is that the amount of seeds used is large, and the amount of seeds used for each spray is about 100mL. And pay attention to reducing the water content of the culture material when mixing. Practice has proved that this method has been successfully applied in the production of shiitake mushrooms.
5. Shaking inoculation method after inoculation
The granular strain culture medium with the liquid strain can best play the advantage of rapid growth. After the liquid strain is connected to the glass bottle, rotate or shake to make the liquid flow and stick to each granule, and it will grow up in 3 days at a suitable temperature. Silk, continue to cultivate for a few days to let the mycelium penetrate into the inside of the particle before it can be used for production. This method can be used when seed is needed urgently.
Liquid strains are not as resistant to adverse environments as solid strains (such as low hypoxia tolerance), and it is often difficult to succeed in cultivation with fermented materials, raw materials or plastic bag clinker open mixing materials. Only a good combination of liquid strain production technology and edible fungus cultivation technology can give full play to the advantages of liquid strains.
The above methods can be used flexibly. For example, in the production of Cordyceps militaris, since the culture medium contains a large number of spores produced by hyphae, as long as the liquid flows to the place during inoculation, there will be hyphae growth, whether it is thin material production fruit body or thick material production fungus Silk powder can be sprayed with liquid strains on the material table.