How to improve the survival rate of shiitake mushroom inoculation
Planting shiitake mushrooms is generally completed by inoculating with fungus sticks. Pay attention to the following aspects when inoculating:
Inoculation site: Select a room that is sheltered from wind, clean, dry, well-sealed, and free of bacterial contamination within 50 meters as an open inoculation site. The inoculation room and inoculation box must be cleaned and disinfected one day before the fungus sticks are brought in.
Inoculation season: Generally, open inoculation is carried out in winter and spring, because the high temperature in summer and autumn can easily cause contamination of the rods and bacteria, and open inoculation cannot be performed. When inoculation is performed in winter, the temperature is low, and open inoculation technology can be used. When inoculating shiitake mushrooms, choose sunny and dry weather, which can effectively improve the survival rate of inoculation.
Strain selection: medium-high temperature varieties with vigorous mycelium production should be selected.
Bacteria stick treatment: Sterilize under normal pressure, and after keeping it at 100°C for 14 hours, move the bacteria stick without mechanical damage to the inoculation room, and cover it with a film to prevent dust and miscellaneous spores from falling. fungus sticks are piled up in the middle of the inoculation room, and the pile height should not exceed 1.2 meters. After the fungus sticks are cooled to below 25°C, they can be inoculated after being disinfected with aerosol disinfectant for 2 hours.
Inoculation procedure: Disinfect the strains and inoculation tools with 75% alcohol for later use, and rub the inoculation site with a disinfectant. Punch holes in the rubbed area, and punch 3 to 4 holes in each section of the fungus stick. The holes are 4 to 5 cm deep and cannot be pierced. Break the production seeds into pieces and stuff them into the holes. The inoculation pieces and the fungus sticks should be combined tightly, but not squeezed, so as to prevent the intrusion of bacteria from the gap. When the temperature rises to 25°C, uncover the film for ventilation, and turn the pile in time to prevent high-temperature burning bacteria.